IL-1 alpha/IL-1F1 Antibody AF-200-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-1 alpha/IL-1F1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF-200-NA
NCBI Gene AliasesIL-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1
ApplicationsWestern Blot, Neutralization, Immunocytochemistry
HostGoat
IsotypeIgG
ReactivityHuman
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
TargetIL-1 alpha/IL-1F1
NCBI Full Gene Nameinterleukin 1 alpha
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human IL-1 alpha/IL-1F1, Ser113-Ala271, Accession # Q53QF9
ConcentrationLYOPH
PurityAntigen Affinity-purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human IL-1 alpha/IL-1F1 in direct ELISAs and Western blots

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) IFI16 Induced by Direct Interaction between Esophageal Squamous Cell Carcinomas and Macrophages Promotes Tumor Progression via Secretion of IL-1αCellsNovember 10, 2023Yuki Azumi, Yu-ichiro Koma, Shuichi Tsukamoto, Yu Kitamura, Nobuaki Ishihara, Keitaro Yamanaka, Takashi Nakanishi, Shoji Miyako, Satoshi Urakami, Kohei Tanigawa, et al.Switzerland). In some experiments, ESCC cells were treated with 1 μg/mL neutralizing antibody against IL-1α (AF-200-NA, R&D Systems) or 1 μg/mL normal goat IgG (AB-108-C, R&D Systems) as the negative control, with or without

(2) Discovery of senolytics using machine learningNature communicationsJune 10, 2023Smer-Barreto V, Quintanilla A, Elliott RJR, Dawson JC, Sun J, Campa VM, Lorente-Macías Á, Unciti-Broceta A, Carragher NO, Acosta JC, et al.for 10 min and then blocked for 30 min with PBS-BSA-Gelatin fish (Sigma). Primary antibodies (IL1A AF-200-NA R&D Systems; IL1B MAB201 R&D Systems and IL8 MAB208 R&D Systems) were prepared in a blocking buffer

(3) Human T(H)17 cells engage gasdermin E pores to release IL-1α on NLRP3 inflammasome activationNature immunologyFebruary 1, 2023Chao YY, Puhach A, Frieser D, Arunkumar M, Lehner L, Seeholzer T, Garcia-Lopez A, van der Wal M, Fibi-Smetana S, Dietschmann A, et al.

(4) Cystathionine-β-synthase is essential for AKT-induced senescence and suppresses the development of gastric cancers with PI3K/AKT activationeLifeJune 27, 2022Zhu H, Chan KT, Huang X, Cerra C, Blake S, Trigos AS, Anderson D, Creek DJ, De Souza DP, Wang X, et al.monoclonal),Cell Signaling Technology,Cat#19689,WB (1:1000) Antibody,Anti-IL-1α (Goat polyclonal),R&D Systems,Cat#AF-200-NA,WB (1:1000) Antibody,Anti-IL-1β (mouse monoclonal),R&D Systems,Cat#MAB201100,WB (1:1000) Antibody,Anti-IL-8

(5) Wu-Zi-Yan-Zong-Wan protects mouse blood-testis barrier from Tripterygium wilfordii Hook. f. multiglycoside-induced disruption by regulating proinflammatory cytokinesJournal of ethnopharmacologyNovember 15, 2021Pan Z, Gao Y, Liu S, Ke Z, Guo J, Ma W, Cui T, Liu B, Zhang X.

(6) Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal AdenocarcinomaBiomedicinesOctober 22, 2020Liu D, Steins A, Klaassen R, van der Zalm AP, Bennink RJ, van Tienhoven G, Besselink MG, Bijlsma MF, van Laarhoven HWM.and 10% Noggin (produced by stably transfected 293T cells) and 10 µg/mL recombinant human anti-IL1α (AF-200-NA, R&D systems, Minneapolis, MN, USA) respectively. 2.1.8. Transwell Migration Assay Migration assays

(7) The Pseudomonas aeruginosa protease LasB directly activates IL-1βEBioMedicineOctober 1, 2020Sun J, LaRock DL, Skowronski EA, Kimmey JM, Olson J, Jiang Z, O'Donoghue AJ, Nizet V, LaRock CN.and IL-1β (200-LA, 201-LB; R&D Systems), and neutralising antibodies for each (A15032A, Biolegend and AF-200-NA, R&D Systems) were used following manufacturer protocol (hkb-il1r; Invivogen) to confirm reporter sensitivity,

(8) Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesisAutophagyJanuary 1, 2017Qiang L, Sample A, Shea CR, Soltani K, Macleod KF, He YY.

(9) Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic PatientsPloS oneJanuary 1, 2016Savill SA, Leitch HF, Harvey JN, Thomas TH.R&D Systems), 500 ng/ml Human TNFα antibody (AF-210-NA, R&D Systems) and 1 μg/ml Human IL-1α antibody (AF-200-NA, R&D Systems). Cells were incubated for 24 hours in treatment conditions before being washed twice in

(10) Tissue and serum immune response in chronic hepatitis C with mild histological lesionsMemórias do Instituto Oswaldo CruzFebruary 1, 2010A T R-Viso et al.The following specific monoclonal antibodies were employed: IFN-γ (MAB285), TNF-α (AF-210-NA), IL-1α (AF-200-NA), IL-1β (AF-201-NA), IL-2 (AF-202-NA), IL-4 (AF-204-NA), IL-6 [AF-206NA and IL-10 (MAB217) (all from

(11) Host Cell Cytokines Induced by Chlamydia pneumoniae Decrease the Expression of Interstitial Collagens and Fibronectin in FibroblastsInfection and ImmunityFebruary 1, 2009Jürgen Baumert, Karl-Hermann Schmidt, Annett Eitner, Eberhard Straube, Jürgen Rödelfibroblast growth factor ([bFGF] AF-00-NA), beta interferon ([IFN-␤] AF-00-NA), or interleukin-1␣ ([IL-1␣] AF-200-NA) (all from R&D Systems, Wiesbaden, Germany) were added to conditioned medium at concentrations of 0.3

(12) P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cellsBritish journal of pharmacologyMay 1, 2007Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC.

(13) The response of human epithelial cells to TNF involves an inducible autocrine cascadeCellMarch 24, 2006Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK.

(14) Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1)CytokineJuly 21, 2005Palmer G, Trolliet S, Talabot-Ayer D, Mézin F, Magne D, Gabay C.

(15) Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkeyCytokineDecember 21, 2003Sengupta J, Dhawan L, Ghosh D.

(16) Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alphaCirculation researchSeptember 19, 2003Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF.

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IL-1 alpha/IL-1F1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (16)

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IL-1 alpha/IL-1F1 Antibody from R&D Systems, a Bio-Techne Brand

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